Collection, Isolation, Media Selection, and Cultivation Protocols for wild Sanghuang Mushroom (Phellinus linteus)

Sanghuang mushrooms (Phellinus sp.), discovered in An Giang province, Vietnam, were initially identified as Phellinus sp. through morphological analysis and ITS sequence comparison, revealing 92% similarity in GenBank. Despite 8% variability, the mushrooms were tentatively classified under the genus Phellinus. The species Sanghuang mushroom (Phellinus linteus), identified through morphological and molecular biology methods, has been named Phellinus linteus AG by the authors. It was collected from the high mountainous region of An Giang Province, Vietnam. This study investigated optimal propagation methods and the cultivation process. Initial propagation on Potato Dextrose Agar (PDA) medium and subsequent propagation on Rice medium + 5% rice bran resulted in hyphal growth within 7 days. To maximize fruiting body yield, a substrate composed of 90% rubber sawdus + 5% rice bran + 5% cornstarch yielded fruiting bodies within 55 days of inoculation after 33 days of substrate colonization. The study also proposed a production process for Sanghuang mushrooms, derived from research on various cultivation media and substrates for fungal growth. The findings contribute to enhancing the understanding of Phellinus linteus cultivation and emphasize the importance of optimizing growth media to enhance the yield and productivity of this valuable fungus.