Growth Rate and Sugar Consumption Analysis of Grey Oyster Mushroom (Pleurotus pulmonarius) Chlamydospore in a Bioreactor

Propagation and cultivation of chlamydospore from spore induction technique had successfully studied to become a promising technique in grey oyster mushroom spawn production. However, an increase volume of this technique is never been tested to ensure large scale ability production. Therefore, a study was conducted to examine the possibility of propagating and cultivating chlamydospore in a 2.5 L stirred tank bioreactor. The inoculums used were a month and 5 months storage of chlamydospore and were compared with the same storage duration of mycelium culture from tissue culture technique. As expected, chlamydospore had showed better performance in term of maximum dry mycelia weight, specific growth rate and doubling time than mycelium spawn. The highest specific growth rate was obtained from 5 months stored chlamydospore at 0.0088 g hour-1 with shortest doubling time 78.75 hours. While the slowest was obtained from 5 months stored mycelium with specific growth rate at 0.0069 g hour-1 and longest doubling time 100.43 hours. The sustainable of active mycelium was verified with the analysis of reducing sugar concentration in the media where sugar uptake was high for chlamydospore at one month and 5 months stored chlamydospore.