Three new species of endophytic nitrogen fixing bacteria and their effects on soil fertility, baby corn yield and their chemical nitrogen fertilizer use efficiency

The relationship between endophytic nitrogen fixing bacteria and nitrogen fertilizer has insignificantly studied in this crop. Therefore, endophytic nitrogen fixing bacteria were studied the N fixing aptitude and their other simulative effects on nitrogen fertilizer reduction and Baby maize yield increase. Three new endophytic nitrogen fixing bacteria consisted of Bacillus sp. strain NTLG2-5, Pseudomonas strain DJ06 and Enterobacter asburiae IIWM-JS-07L, which were isolated and identified from the baby maize roots, had high homogeneous rates (100, 99.92 and 99.63, respectively). Results proclaimed that 50% reduction of nitrogen fertilizer application and three endophytic nitrogen fixing bacteria improved significantly on farmland nutrition and increased agronomic, yield components, productivity and quality of baby corn. The 50% reduction of nitrogen fertilizer fertilization, the edible cob yield increased up to 61.7% compared to without nitrogen fertilizer application and was not adequately different to compare to application of 100% nitrogen fertilizer. Furthermore, inoculation of Bacillus sp. strain NTLG2-5, Pseudomonas strain DJ06 and Enterobacter asburiae IIWM-JS-07L obtained edible yields that were 23.1%, 54.1%, and 64.4%, respectively, higher than without inoculation. Inoculation of Enterobacter asburiae IIWM-JS-07L species attained the highest edible cob yield (5.50 t/ha) and Bacillus sp. strain NTLG2-5 inoculation had the lowest yield (2.55 t ha-1). All three new species, especially Enterobacter asburiae IIWM-JS-07L species, have the best ability to decrease nitrogen fertilizer application and increase soil fertility and baby corn yields.